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Dr. Muhammad Baqir MR Fakhrildin
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*Israa Hussein Hamzah, Dr. Abdul Ameer N. Al-Rikabi, Dr. Abdul Hussein M. Al- Faisal, and Dr. Adel R. Al-Saadawi MD


Background: Hodgkin’s lymphoma is type of lymphoid malignancy, it’s an unusual cancer because the neoplasm cells constitute only a minority of the total tumor mass, and the cause of developing disease might be attributed to the infectious agent such as virus especially EBV which contributes to the development of Hodgkin’s disease in some cases. Recently the pathogenesis of Hodgkin’s disease is studied by new technologies applications like conventional PCR and gene expression array analysis. EBV may be play direct or indirect role in the pathogenesis of disease which due to the trigger some mechanism(s) or May due to the presence of an inherited or acquired depression of immunoregulation that effect on the development of disease. Method: The study was aimed to detect the BNRF1 gene of EBV in Hodgkin’s lymphoma Iraqi patients. The study include 75 Iraqi patients with Hodgkin’s lymphoma (25 samples as blood and 50 samples as formalin fixed paraffin embedded (FFPE) tissue blocks) and 10 samples as reactive hyperplasia blocks for control. The blood samples were collected from hematology unit in Baghdad hospital in medical city but paraffin blocks tissue were collected from histopathological laboratories in different hospitals during the period from September 2013 till March 2014. The DNA was extracted from all samples by using specific kit and used in the experiments of this study. The work was carried out in the molecular oncology unit lab of GSTS pathology Guy’s and St. Thomas’ NHS foundation Trust Hospital /London/United Kingdom. The DNA which was extracted before was used to detect the existence of BNRF1 gene specific for EBV loaded in patient samples by using real time quantitation PCR (qRT-PCR) with specific primer and probe. Results: The EBV DNA genome encoding the non- glycosylated membrane protein (BNRF1) was detected in 40(53.33%) positive cases of 75 Hodgkin’s lymphoma samples and no cases positive for EBV in reactive hyperplasia with high significant (P<0.01**) . The majority of positive patients Were males 33(82.5%) than females. In addition to the EBV was more frequently detected in mixed cellularity subtype of HL 27(67.5%). Conclusion: The QC- PCR assay allows accurate quantification of EBV load and show as a tool to assist in diagnosis and management of EBV related lymphoma patient and cancer related with EBV DNA that may be considered as a tumor biomarker.

Keywords: EBV, Hodgkin lymphoma Iraqi patients, RT-PCR.

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