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Abstract

PHOTODYNAMIC THERAPY CAN KILL SEVERAL CANDIDA SPECIES IN IN VITRO AND IN VIVO MODELS

Amal A.I. Mekawey, Mona E. Morsy and Hanaa Y. A. Abdou

ABSTRACT

Candidiasis is an infection caused by the encapsulated yeast Candida species and the most afflicted sites are skin, lung, renal and nervous systems. The aim of this study was to investigate the ability of methylene blue (MB) combined with a low-power red laser to inactivate four Candida species in in vitro and in vivo experimental models. To perform the in vitro study, suspension of Candida tested (106cfu/mL) was used. The light source was a laser (diode laser with wavelength 660 nm) (Photon Lase III, DMC, Sao Carlos, Brazil) emitting at 660nm with output power of 200 and 250mW for 5 and 10 min of irradiation, resulting fluences of 200 mw/cm2 equal 60 and 120 J/cm2 at 5 and 10 min respectively as well as 250 mw/cm2 had fluences at 5 and 10 min equal 75 and 150 J/cm2, respectively. As photosensitizer, methylene blue (MB) 0.1 mg/ml was used. For the in vivo study, Twenty male Wistar rats (Rattus norvegicus) inoculated with the most effect Candida species (106cfu/mL). Twenty-four hours after inoculation, PDT was performed using MB and the laser power exhibited the most effective on Candida species. Histopathology of mice wound tissue was examined after 3 , 5 and 7 days. PDT was efficient in vitro against Candida species. In the in vivo experiment, PDT was also effective; however, its effect was less expressive than in the in vitro study. In conclusion, PDT seems to be a helpful alternative to treat dermal candidiasis; however, more effective parameters must be found in in vivo studies.

Keywords: Antifungal; Candida; Diode laser; Methylene Blue; Photodynamic therapy; Photosensitizers.


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