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Abstract

EFFECT OF COLCHICINE INDUCED MUTATION ON CELLULASE ENZYME PRODUCTION BY ASPERGILLUS FUMIGATUS

Dr. Johra Khan*, Dr. Amal Alotaibi and Dr. Manab Deka

ABSTRACT

The production of cellulase before and after colchicine induced mutation was investigated in shake flask culture condition. Aspergillus fumigatus was isolated from different agriculture wastes. The enzymatic scarification of cellulase was proposed as a mean of producing glucose on industrial scale. In colchicine treated species it was found possible to increase the production of C1 up to 40 folds in 0.1% concentration, 90 fold increases in CX production, 95 fold increases in filter paper production and 96 fold increase in β- glucosidase activity. The effect of colchicine mutation on cellulase production was studied in 0.1% and 0.2% concentration. The effect of colchicine was examined in Mendal and Sternberg medium (1976) which shows that 0.1% concentration can cause significant increase in the production of cellulase for industrial production.

Keywords: Aspergillus fumigatus, Colchicine, Mutation, Cellulase enzyme, Cellulose


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