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Abstract

DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR DETERMINATION OF VENLAFAXINE HCL IN HUMAN PLASMA.

Manisha Phoujdar*, Snehal Maske, Shwetal Vasa and Abhijeet Shahane

ABSTRACT

A rapid and sensitive RP-HPLC method has been developed and validated for quantification of Venlafaxine hydrochloride in human plasma. The human plasma samples were extracted by using anhydrous diethyl ether. AlltimaTM C8 column provided chromatographic separation of the analyte. The proposed method has been validated with linear range of 2-25 ng mL for Venlafaxine hcl. The precision and accuracy values are within 10%. Total elution time was 10 min. The all validation parameters lie within the acceptable limits.

Keywords: Venlafaxine hcl, Human plasma.


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