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Abstract

SCREENING OF PHYTOCHEMICAL AND IN VITRO ANTIOXIDANT PROPERTY OF N-MIRACLE (POLYHERBAL FORMULATION).

Meenatchi Sundaram Angappan* and Jeyaprakash Karuppaiah

ABSTRACT

Antioxidants are substances that inhibit oxidation, and are capable of counteracting the damaging effects of oxidation in body tissue. They prevent damage caused by free radicals. They create a barrier from free radial damage that results in decaying process of oxidation. Under most pathological conditions there is generation of reactive oxygen species and other free radicals. Polyherbal compounds are concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard. The investigation of phytochemical compounds of the material extracted in ethanol, methanol and water revealed the presence of saponins, flavonoids, steroids, terpenoids, triterpenoids, alkaloids, carbohydrates, anthraquinone, phenolic compounds, and glycosides. Antioxidant activity of the N-Miracle (Polyherbal formulation) was also assessed by DPPH assay, total antioxidant assay, superoxide radical scavenging assay, and reducing power assay. They showed commendable antioxidant effect as that of the ascorbic acid. The antioxidant properties were due to the presence of promising phytoconstituents in N-Miracle (polyherbal formulation).

Keywords: Antioxidants, Reactive Oxygen Species, N-Miracle, Phytoconstituents, Polyherbal formulation.


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