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Abstract

ISOLATION AND SCREENING OF ENDOPHYTIC BACTERIA FROM RICINUS COMMUNIS FOR ANTIMICROBIAL ACTIVITY

Pranay Jain* and Priyanka Sharma

ABSTRACT

The present study deals with the isolation of endophytic bacteria from the leaves, stem and beans of Ricinus communis plant and its screening for antimicrobial activity against twelve test pathogens viz. Streptococcus mutans (MTCC 497), Streptococcus pyogenes (MTCC 1924), Staphylococcus aureus (MTCC 7443), B. megaterim (MTCC 428), Bacillus subtilis (MTCC 121), Escherichia coli (MTCC 40), Pseudomonas fluorescens (MTCC 1748), Candida albicans (MTCC 227) Candida tropicalis (MTCC 3421), Candida glabrata (MTCC 3814), Alternaria solani (MTCC 10690) and Fusarium graminearum (MTCC 2089). Out of twelve test pathogens, the endophytic bacterial isolate inhibited the activity of eight test pathogens. The minimum inhibitory concentration of ethyl acetate extract of antimicrobial metabolite ranged from 12.207μg/ml to 25 mg/ml.

Keywords: Endophytic bacteria, pathogens, antimicrobial activity, minimum inhibitory concentration.


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