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World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
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Abstract

EVALUATION OF MICRORNA-21 IN INVASIVE DUCTAL CARCINOMA IN COMPARISON TO CLINICOPATHOLOGICAL STATUS BY STEM-LOOP RT-PCR PROCEDURE.

Shoroq Mohammed Al-Temimi*, Ali Hausin Al-Khafaj, Salim Rashid Al-Aubaidy.

ABSTRACT

Background:- MiR-21 was one of the first oncogenic miRs and as an anti-apoptotic factor, to be characterized, being up-regulated in numerous tumors including BC . A novel miR quantification method has been established using stem–loop RT followed by TaqMan PCR analysis in tissues or culture cells. Aim of study:-Estimation of miR- 21 expression level in fresh tissues of BC / NATs by using stem-loop follow by TagMan real time PCR (RT-PCR) technique and correlate miR-21 gene expression with age of patients and protein expression of ER,PR and HER-2 . Material and methods:- Stem-loop RT-PCR was performed to identify the expression level of miR-21 in 50 IDC samples and their NATs. IHC for ER,PR and HER-2 . Results:- Mean cancer tissue fold change of miR-21 was significantly higher than that of NATs. Majority of cases showed up regulation of miR-21, 96%. No statistical significant associated with age of patients ,protein expression of ER,PR and HER-2 and fold change of miR-21 expression . Conclusion:- Up-regulation of miR-21 expression has been no statistical significant associated with age of patients and protein expression of ER,PR and HER-2.

Keywords: breast cancer,miR-21gene expression ,stem-loop RT-PCR.


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