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Abstract

STUDY THE MUTAGENIC EFFECTS OF ULTRAVIOLET (UV) ON VIABILITY AND ANTIBIOTIC SENSITIVITY OF ENTEROPATHOGENIC ESCHERICHIA COLI ISOLATED FROM INFANTS DIARRHEA

Bushra H.Saleh*, Reem N. Ibrahim**, Ruqaia Mohammed Ibrahim.

ABSTRACT

During this study a total of (28) diarrheal samples were collected from infants and young children younger than (3) years in (2) hospitals in Baghdad, during the period from 1/4/2014 to 30/8/2014.Diagnosis of Escherichia coli is depends upon isolation and laboratory identification of the bacterium. Bacterial isolates were identified by gram stain and API 20E system. Results showed that among the total of (28) diarrheal samples that were collected, only (19) isolate (67.85%) were gave typical morphological characteristics and biochemical test that related to Escherichia coli while the rest (9) isolates may belong to other pathogenic bacteria from different genera. Results of Serotyping reveals that only (17) isolates from (19) isolates were give positive results with polyvalent antisera on slide agglutination test. Results of antibiotic sensitivity test for (10) EPEC isolates reveals that EPEC isolates were 100% sensitive to trimethoprim/ sulphamethoxazole, ciprofloxacin and gentamycine, 80% of isolates were sensitive to Nalidixic acid and amoxicillin. Results also reveals that 90% of isolates are resistant to ampicillin, bacitracin and Erythromycine. Results of mutation to Escherichia coli isolates (E1) reveals that exposure to UV irradiation from (1-5) mint leads to change in the sensitivity of isolates to, amoxicillin, chloramphenicol ,gentamicin and trimethoprim/ sulphamethoxazole from (25,24,20,20) mm zone of inhibition respectively to resistant isolates while the same isolate reveal changes in results from resistant to sensitive to cefixime and bacitracin which have (22,20) mm zone of inhibition respectively after mutation but the same results were remained for the other antibiotic (ciprofloxacin, Erythromycin, ampicillin, nitrofurantion, and Naldixic acid. Also results of mutation to Escherichia coli (E1) isolates reveals that exposure to UV irradiation from (1-5) mint leads to change in the viability of E1 isolates in which increased exposure time to UV irradiation leads to decrease in viable cell count from too numerous to count in control plate to (80x106), (50x106)CFU/ml in M1,M2 plates after exposure to UV light for 1,2 mint, then increased exposure time to (3,4,5)mint lead to decrease viable cell count to (25,14,2) x 106 CFU/ml in (M3,M4,M5) plates respectively.

Keywords: Bacterial isolates, UV irradiation, sulphamethoxazole.


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