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Abstract

METHOD DEVELOPMENT AND VALIDATION OF MOXIDECTIN IN SYNTHETIC MIXTURE USING UV-SPECTROPHOTOMETRY

P Sathish Babu, K. Anand Babu*, D Revathi and K Chitra

ABSTRACT

A sensitive, accurate and validated UV Spectrophotometry method has been developed to determine Moxidectin in bulk drug and synthetic mixture. The Calibration graph was plotted over the range of 8-22 μg/ml with correlation coefficient value of 0.9994. The Limit of Detection (LOD) and Limit of Quantification (LOQ) for Moxidectin were 0.0264 and 0.08 μg/ml. The percentage RSD for precision of the method was found to be less than 2%. The assay percentage was found to be 100.8%. The newly developed method was validated according to the ICH guidelines with respect to linearity, accuracy, precision and specificity.

Keywords: Moxidectin, Validation, Synthetic mixture, ICH Guidelines.


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