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Abstract

DEVELOPMENT AND VALIDATION OF UVSPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF LEVOFLOXACIN HEMIHYDRATE IN BULK AND MARKETED DOSAGE FORMS

Deokar G.S.* and Borate S.R.

ABSTRACT

A simple, rapid, accurate and economical u-v spectrophotometric method have been developed using solvent 0.1 N HCL for the determination of levofloxacin hemihydrate in bulk and pharmaceutical dosage formulations (specially floating dosage form). At predetermined absorption maxima (╬╗max) of 293nm, it was proved linear in range 2-12ug/ml and exhibited correlation coefficient 0.998 Method was validated by following the analytical performance parameters suggested by the International Conference on Harmonization (ICH). All validation parameters were within the acceptable range. The obtained results proved that the method can be employed for the routine analysis of levofloxacin hemihydrate in bulks as well as in the commercial formulations.

Keywords: Levofloxacin hemihydrate, UV-Spectrophotometricmethod, Validation, Estimation.


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