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Abstract

DEVELOPMENT OF HPTLC METHOD FOR THE DETERMINATION OF PIPERINE IN LAVANGADI VATI – AN AYURVEDIC FORMULATION

Shivani1, Iram rakhshi2,  Pawar R.K* 3, Sharma Shivani 4, Sharma Rajeev K.R. 5 and Singh K.C. 6

3,5Pharmacopoeial Laboratory for Indian Medicine, Ghaziabad 201 002 (U.P.), India.
1,2,4,6Department of chemistry, R.S.S. (P.G.) College, Pilkhuwa, Hapur (U.P.), India.

ABSTRACT

A simple, rapid, selective and quantitative HPTLC method has been developed for determination of Piperine in Ayurvedic formulations of Lavangadi vati of different manufactures. The alcoholic extract of Lavangadi vati and Pippali fruit samples were applied on TLC Aluminium plate pre coated with Silicagel60 GF254 and developed using Toluene : Ethyl acetate (9:1) v/v as a mobile phase. The plate was sprayed (derivatized) with Anisaldehyde‐Sulphuric Acid reagent followed by heating at 1100C for 10 minutes and detection and quantification were carried out densitometrically using anUV detector at wavelength of 254 nm. Content of marker compound in the samples were found similar.

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