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Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF PAZUFLOXACIN MESYLATE IN PHARMACEUTICAL DOSAGE FORM

Mitesh R. Gaonkar* and Teja V. Walke

ABSTRACT

The present work involves an attempt to develop a precise and accurate UV spectrophotometric method with greater sensitivity, reproducibility for the estimation of Pazufloxacin mesylate in pure and pharmaceutical dosage form. Pazufloxacin mesylate is available in infusion dosage form for its medical use. Pazufloxacin mesylate shows λmax of 247 nm in 0.1N HCl. The developed method was found to show linearity in concentration range of 2-12 μg/ml with the value of correlation coefficient (R2) 0.9905. The percent Relative Standard Deviation (%RSD) showed during inter and intra-day precision studies were also found to be within limits of nmt 2% concluding that the present method is precise as per ICH guidelines Q2 (R1).[1] The developed method can be used for routine estimation of Pazufloxacin mesylate in bulk and pharmaceutical dosage form.

Keywords: UV spectrophotometry, Pazufloxacin Mesylate, Absorbance, Absorptivity.


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