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Abstract

ISOLATION, CHARACTERISATION AND QUANTIFICATION OF EXTRACTED D-PINITOL FROM BOUGAINVILLEA SPECTABILIS STEM BARK

Mininath Vidhate*, Arati Ranade, Bhagvat Vidhate, Pallavi Birajdar

ABSTRACT

Bougainvillea spectabilis is a most abundantly found plant having an insulin mimicking agent D-Pinitol (3-o-methyl-chiroinositol). The aim of the present study was to extract, isolate and characterize D-Pinitol from Bougainvillea spectabilis stem bark. D-Pinitol extraction from Bougainvillea spectabilis was processed with ethanolic extraction followed by isolation and separation using silica gel column chromatography. This was followed by recrystallisation using methanol. D-Pinitol isolated from the plant was identified on the basis of Infrared Spectroscopy (IR), Nuclear Magnetic Resonance Spectroscopy (NMR), Differential scanning calorimetry (DSC) and Thin Layer Chromatography (TLC) as 1-methoxy-2,3,4,5,6- pentahydroxy cyclohexane. For the purpose of quantifying Ultraviolet Spectroscopy (UV) and High performance liquid chromatography (HPLC) methods were developed and validated.

Keywords: Bougainvillea spectabilis, recrystallization, liquid chromatography.


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