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Abstract

SCREENING OF MULTI DRUG RESISTANT BACTERIA AND FUNGI FROM MEDICAL DEVICE USED IN URINARY TRACT INFECTION AND INFLAMMATION IN HOSPITAL OF GUNTUR DISTRICT, A.P, INDIA.

Mostafa Mohammed Atiyah* and Amrutha V. Audipudi

ABSTRACT

The ability to stick to materials and promote the formation of biofilm is an important feature of bacteria resulting from participation in the foreign body infections. The fact that staphylococci are the main things related injury medical devices has stimulated a lot of research on the pathogenic mechanisms, which led to significant achievements in our understanding of biofilm formation. And so on, it has been identified on the battery of Staphylococcus virulence factors, and characterized in the past two decades, leading to important information, particularly with regard to bacteria interact with the surface of the transplanted organ or inserted as normal, and live in harmony cons balanced on our skin, and which constitute a key element small plants coetaneous. Put out this medical device objects rarely causes infections. With this, the development needed to study living organism’s goals current high living and multiple resistance responsible for community acquired revenues and UIT hospital obtained by catheterization for patients. was observed growth (18) species of bacteria in alleviating serial dilution concentration (104) (AVMP 1) were distributed among bacteria (E.coli) and the number (7) and percentage (38.8%), and bacteria Pseudomonas aeruginosa The number (3) and the percentage (16.6%), and bacteria (Proteus mirabilis) was the (2) and percentage (11.1%), and bacteria (Bacillus ceruse) and the number (4) and percentage (22.2%), and bacteria (Citrobacteria freundi) was the (2) and percentage (11.1%), while the observed growth (15) species of bacteria in serial dilution concentration (105 ) (AVMP 2), distributed among bacteria (Klebsiella pneumonia) and the number (2) and percentage (13.3%), and bacteria (Staph aureus) and the number (5) and the proportion of percentage (33.3%), and bacteria (Staph epidermis) the number (3) and the percentage (20%), and bacteria (Enterococci faecalis) was of (1) and percentage (6.6%), and bacteria (Entrobateria aerogenous) was the (2) and the percentage (3.13%), and bacteria (Bacillus stubilis) was the (2) and percentage (3.13%). It was also noted in this study, the growth of (29) species of fungi which were distributed between Aspergillus Niger and number (6) and percentage (20.6%) yellow and Aspergillus, and the number (8) and percentage (27.5%), and Pencillium prosecution of (5) and the percentage (it was 17.2%), while Candida prosecution of (6) and percentage (20.6%), while the number of Candida albicans (4) and the percentage (13.7%), is also an indication that the medical device also contribute to increase the frequency of microbes.. It was examined antibiotic resistance and (AVMP1, AVMP2) by agar well diffusion method against 10 antibiotics (ampicillin, streptomycin, tetracycline, Chloramphenicol Norfloxacin, aggentomycin, ciprofloxacin, erythromycin, amoxicillin and cefluexcin). Bacterial colony usually occurs in all dilutions ranging from 10-1 to 10-4 and look like a colony isolated one in the eye of 104 mitigation as AVMP 1. And screened for resistance to antibiotics. AVMP1 highly sensitive six antibiotics (such as ampicillin, tetracycline, Norfloxacin, ciprofloxacin, amoxicillin and cefluexacin). While highly resistant to (streptomycin, chloramphenicol, aggentomycin and erythromycin). Bacterial colony usually occurs in all dilutions ranging from 10-1 to 10-5 and look like a colony isolated one in the eye of (105) AVMP2 been isolated in solitary colony 105 also ease the tension of the sensitivity of antibiotics examination. However, ciprofloxacin was AVMP2 very sensitive to all the other multiple antibiotics, anti-Lama (erythromycin, amoxicillin and cefluexacin, Ampicillin, streptomycin, tetracycline, chloramphenicol Norfloxacin, aggentomycin). Life was very resistant to antibiotics , according to the literature and isolate any bacteria that show resistance to more than one strain of bacteria can be formulated resistance. Also on our results to determine AVMP1 as multi-resistant bacteria and the drugs AVMP2 of drug-resistant bacteria and one strain. As for antibiotics fungus takes five antifungal (Clotronazole, Terbin, Ivenmycin, Ketoconazole, Fluconazole), and was examined antibiotic resistance against fungi isolated from medical devices that are used in urinary tract infection and sample (Candida prosecutors) in the serial dilution described 104 and sample It was Aspergillus prosecutors in mitigation serial 10 5 between the two types resistant to all antibiotics fungal used in this study, either with respect to pencillium mitigation serial in 103 has a couple of antibiotic resistance built are (Ketoconazole and Fluconazole) while three antibiotics sensitive are (Terbin Clotronazole, Ivenmycin), it is assumed that the management of persistent antibiotics to the hospital received patients with urinary tract infection and confirm the catheter young plants resist infection to join the medical device.

Keywords: Screening bacteria and fungi from medical device used IN UTI , antibacterial agent and antifungal activity.


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