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Abstract

DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF MEBENDAZOLE IN BULK AND PHARMACEUTICAL FORMULATION

Durgesh Rameshlal Parakh*, Moreshwar P. Patil, Sandeep S. Sonawane, Chetan P. Jain

ABSTRACT

A new simple and sensitive spectrophotometric method in UV region has been developed for the determination of Mebendazole in bulk and in pharmaceutical formulations. Mebendazole exhibits absorption maxima at 234 nm. The method obeys the Beer’s law in the concentration range of 1-10 μg/mL. The method is accurate, precise and economical. The percent recovery is near to 100%. This shows that the method was free from the interference of excipients. The results of validation study were analyzed with respect to accuracy, limit of detection, linearity, limit of quantification, precision, ruggedness, robustness and specificity were found to be satisfactory. The proposed method has been applied successfully of drug in bulk and pharmaceutical formulation.

Keywords: Mebendazole, Anthelmintic, UV-Visible spectroscopy, Validation, Recovery.


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