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*Saad S. Al-Dujaily,PhD and Wafaa M. Al-Jubori,MD. DGO


Background: Many centers worldwide used Ericsson method for gender selection. The studies found that sperm with high concentrations of either X or Y bearing sperm could be obtained. Objective: To examine the effect of modified procedure of Ericsson method on gender selection following intra-uterine insemination (IUI) for infertile couples. Patients, Materials and Methods: fifty infertile couples were included in this study. They were randomly divided into two groups Group one , the gender selection protocol was done by discontinuous density gradient(45% and 90%) technique with 7% and 17% albumin concentration technique. Group two simple layer and density gradient techniques were used for gender selection. Then IUI was accomplished and pregnancy test was done following 14 days of insemination to detect the level of h CG. The gender of fetus was obtained by ultrasonography examination from sixteen weeks gestation onward. Results: There was a highly significant (P<0.01) improvement in the percentage of sperm motility grade A and B after the activation was recorded after in vitro activation by density gradient and albumin concentration techniques compared with simple layer and density gradient techniques. The percentage of morphologically normal sperm following in vitro activation was revealed no significant (P>0.05) differences between the two techniques. There was 10 out of fifty women become pregnant (220%). Distribution of live birth babies according to gender using protocol of group two was 6 male babies and the percentage of male sex selection was (66.6%), while only 3 female babies delivered which give a percentage of (33.4%). Conclusion: It is concluded that using density gradient and albumin techniques is more effective than simple layer and density gradient techniques for gender selection when infertile couples seek to have a baby by IUI.

Keywords: Gender selection IUI, density gradient, albumin columns.

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