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Abstract

DETECTION OF VIRULENCE GENES(HLY,FIMH,KAPSMII) IN ESCHERICHIA COLI ISOLATES FROM BETA-THALASEMIC AND NON THALASEMIC PATIENTS BY USING PCR TECHINQUE

Shahad Raad Mezaal*, Ayad Al-Ubaidy and Zaid N. Abbas

ABSTRACT

Background: Thalassemia is a hereditary anemia resulting from defects in hemoglobin production. Beta Thalassemia, caused by a decrease in the production of β-globin chains, affects multiple organs and associated with considerable morbidity and mortality. This study aimed to comparsion between thalasemic and non thalasemic by used specific primers and identifying different microorganisms (aerobicand anaerobic) from urine and blood to detect. Materials and methods: The study samples consist of[41] Thalasemic and[18] non Thalasemic patients respectly. Isolation from mid urine and[25] Thalasmic and [25] non Thalasemic patients respectly. Isolation from Blood. Results: The main bacterial isolates were identified as47 isolates of E.coli (43.1 %), 9 isolates (8.3%) of S. Pneumonia., was 8 isolates of C. Pneumonia (7.3 %), 3 isolates of porteus and K. Pneumonia1 (11.9%). Whereas 6 isolates of Enterobacter colaca represented (5.5%), While P. aeruginosa represente 9 (8.3 %) and lastly Salmonella typhi and pseudomonas .spp(3.7%) for each In addition, Pantoea spp represents (2.8%). A highly significant difference was found between thalassaemic patients and non thalasemic found virulence genes. Bacterial in urine and blood of were found to be higher in the study compared to control group and the difference was statistically highly significant(P<0.01). Molecular methods (PCR analysis)of virulence genes ,resistance,genotyping of E.coli by using primers specific to type 1 fimbria gene (fimH) and capsule gene (kpsMTII), hemolysin (hly). The PCR assay results identified 45 fimH (58%) and kpsMTII (40%) while hly (28%). Conclusion: Patients with Beta thalasemic major had more infectionsseverity compared to non Thalasemicpitients.

Keywords: KEYWORDS: ?-thalassemia, Escherichia coli.


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