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Abstract

EVALUATION OF DNA-PLOIDY AND S-PHASE FRACTION ANALYSIS BY FLOW CYTOMETRY IN HUMAN BREAST CARCINOMA.

Pushpa Rani. V*

ABSTRACT

Breast cancer is the most common form of malignancy that affects women. Tumor proliferation can be monitored by measuring DNA synthesis using flowcytometry which provides rapid and precise analysis of large numbers of cells. The aim of the present study was to evaluate DNA ploidy in breast cancer. A number of 85 patients diagnosed with mammary carcinoma and subjected to surgery and different hospital were taken into consideration. None of the patients were subjected to chemo- or radio-therapy prior to surgery. Cell cycle analyses were performed with fresh tumour sample and normal tissue surrounding the tumour. The present study investigate flowcytometric histogram revealed that 25% of the tumour samples showed 62.99% of cells in S phase that were hypodiploid, 25% of subjects with a value of 31.89% were aneuploid and 50% showed a value of 31.89% were aneuploid. Besides, 50% of the samples indicated a S phase value ranging from 9%-20% and were deemed diploid. Data were analysed by the ModFit LT3.1 software provided by Becton Dickinson. Our study indicated that DNA ploidy could be an important factor for estimating the degree of genomic instability which may be reflected by the aggressiveness of the tumor.

Keywords: Breast Cancer, Flowcytometer, S-phase Fraction, DNA- ploidy.


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