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Abstract

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DIACEREIN AND GLUCOSAMINESAMINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

T. Sriveena*, A. Srividya, A. Ajitha and V. Uma Maheswara Rao

ABSTRACT

A simple, precise, Rapid, Specific and accurate reverse phase high performance liquid chromatography method was develop for simultaneous estimation of Diacerein and Glucosamine in pharmaceutical dosage form. A chromatographic condition was performed on ODS (C18) (4.6×250mm, 5μ) column, with mobile phase is Triethylamine pH4: Methanol: Acetonitrile in the ratio of 50:10:40 % (v/v), at the flow rate 1ml/min at detection wavelength of 250nm. The retention times of Diacerein and Glucosamine were found to be 2.242 and 3.678mins respectively with a run time of 6min, theoretical levels for Diacerein and Glucosamine were 8596 and 4547 respectively. As per ICH guidelines the method was validated for linearity, accuracy, and precision, limit of detection and limit of quantification, and ruggedness, robustness. Linearity of Glucosamine was found in the range of 75-375μg/ml and that for DIA was found to be 5-25μg/ml. The LOD values for Diacerein and Glucosamine were 19.3μg/ml and 1.05μg/ml respectively. The LOQ values for Diacerein and Glucosamine were 58.6μg/ml and 3.19μg/ml respectively.

Keywords: Glucosamine, Diacerein, PDA detection Tablet dosage forms.


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