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Abstract

SIMULTANEOUS METHOD DEVELOPMENT AND VALIDATION OF CITICOLINE AND PIRACETAM IN BULK AND ITS TABLET DOSAGE FORM

Y. Samatha*, A. Sri Vidya A. Ajitha and V. Uma maheswararao

ABSTRACT

A rapid and precise reverse phase high performance liquid
chromatographic method has been developed for the validated of
Citicoline and piracetam, in its pure form as well as in tablet dosage
form. Chromatography was carried out on a XBridge C18 (4.6 x
250mm, 5μm) column using a mixture of Methanol and Phosphate
buffer pH3 (85:15 v/v) as the mobile phase at a flow rate of 1.0ml/min,
the detection was carried out at 229nm. The retention time of the
Citicoline & Piracetam was 1.933, 3.396 min respectively. The method
produce linear responses in the concentration range of Citicoline- 12.5-
62.5, and 5-25mg/ml of piracetam. The method precision for the
determination of assay was below 2.0%RSD. The method is useful in
the quality control of bulk and pharmaceutical formulations.

Keywords: Citicoline & piracetam, RP-HPLC, validation. ; Tablet Dosage forms.


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