WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

DETECTION OF VIRULENCE GENES(EAE,BLACMY-2,BOX) IN ESCHERICHIA COLI ISOLATES FROM BETA-THALASEMIC AND NON THALASEMIC PATIENTS BY USING PCR TECHINQUE.

*Shahad Raad Mezaal, Ayad Al-Ubaidy and Mohammed H. Wali

ABSTRACT

Background: Thalassemia is a hereditary anemia resulting from defects in hemoglobin production. Beta thalassemia, caused by a decrease in the production of β-globin chains, affects multiple organs and associated with considerable morbidity and mortality. This study aimed to comparsion between thalasemic and non thalasemic by used specific primers. And identifying different microorganisms (aerobicand anaerobic) from urine and blood to detect. Materials & methods: The study samples consist of (41) thalassemic and (18 )non thalassemic patients respectly.isolation from mid urine and (25) thalassmic and (25) non thalassemic patients respectively. Isolation from Blood. Results: The main bacterial isolates were identified as47 isolates of E.coli (43.1%), 9 isolates (8.3%) of Sterptococcus pneumonia., was 8 isolates of Chalymidia pneumonia (7.3 %), 3 isolates of porteus spp and Klebsiella pneumonia(11.9%). Whereas 6 isolates of Enterobacter colaca represented (5.5%), While Pseudomonas aeruginosa represente 9 (8.3 %) and lastly Salmonella typhi and pseudomonas .spp (3.7%) for each In addition, Pantoea spp represents (2.8%). A highly significant difference was found between thalassaemic patients and non thalassemic found virulence genes. Bacterial in urine and blood of were found to be higher in the study compared to control group and the difference was statistically highly significant (P<0.01). Molecular methods (PCR analysis) of virulence genes ,resistance,genotyping of E.coli by using primers specific to intimin(eae,cefatriaxone(blaCMY2) the PCR assay results eae ( 25%), blaCMY2 (83%). while (7.5%) appeared allof genes E.coli.finaly box-pcr genotyping was 80%. Conclusion: Patients with Beta thalassem ic major had more infections severity compared to non thalassemic patients.

Keywords: ?-thalassemia, Escherichia coli.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More