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Abstract

DEVELOPMENT OF VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF FOSFESTROL IN BULK AND PHARMACEUTICAL DOSAGE FORM

Siddharth M. Patil*, Sunil T.Galatage, Anil B.Patil

Department of Pharmaceutics, KLE’S College of Pharmacy, Nippani Rajiv Gandhi University of Health Scince, Karnataka, India.

ABSTRACT

In this present research work we developed a validated UV spectrometric method for Fosfestrol. It is simple, fast, accurate, cost efficient and reproducible spectrophotometric method, developed for the estimation of Fosfestrol in bulk and pharmaceutical dosage form. Based on measurement of of absorption of UV light, the spectra of Fosfestrol in methanol and water 1:1 ratio showed maximum absorption wavelength (􀀀max) at 243 nm. The calibration curve was plotted over the concentration range from 5-40 μg/ml of Fosfestrol with correlation coefficient 0.999. Validation was performed as per ICH Q2 guidelines for linearity, accuracy, precision, and recovery. This method has good reproducibility with % RSD less than one. The limit of detection (LOD) and limit of quantification were found to be 0.07352 and 0.2228 respectively by simple UV spectroscopy. Thus proposed method can successfully applied for Fosfestrol in routine analysis work.

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