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Abstract

HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (HILIC) AND ITS APPLICATION IN PHARMACEUTICAL ANALYSIS

Subarna Ganguli*, Tara Sankar Maity, Sruti Bagchi Ghosh, Nilanjan Pahari

ABSTRACT

Alpert coined the term,‟Hydrophilic interaction chromatography” in 1990. This mode of chromatography dates back to earliest days of liquid chromatography. It happened when two scientists namely, Martin and Synge separated amino acids. They separated amino acids on a silica column. Water saturated chloroform as mobile phase was used by them. These scientists explained the separation mechanism as partition of the solutes between a water layer held onto the column surface and chloroform. HILIC as a complex system involves partition, polar as well as ion-exchange interactions.Partitioning occurs due to phase transfer of polar analytes from an organic rich mobile phase into an adsorbed layer of water on the stationary phase.Polar interactions occur between the active surface or ligands of the stationary phase.Ionic interactions occur between charged analytes and oppositely charged moieties on the stationary phase.Based on these factors, HILIC is a preferred technique for polar substances. Therefore, HILIC mode of LC is gaining popularity in the analysis of polar compounds of Pharmaceutical interest and also in the study of metabolomics. Therefore, HILIC mode of LC with its advantages over RP chromatography in the analysis polar compounds of pharmaceutical interest is presented.

Keywords: ?Hydrophilic interaction chromatography”.


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