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Abstract

SYNTHESIS AND CHARACTERIZATION OF CYTOTOXIC SILVER NANOPARTICLES USING MARINE BROWN SEAWEED SARGASSUM JOHNSTONII SETCHELL & N.L.GARDNER

Chandra Kala P, Johnson M*, Shibila T, Revathy I

ABSTRACT

The present study was aimed to optimize the protocol for the synthesis of silver nanoparticles using aqueous extracts of Sargassum johnstonii Setchell & N.L.Gardner and evaluate cytotoxic potentials using brine shrimp bio-assay activity and Trypan blue dye exclusion method. The reduction of pure Ag+ ions was monitored by measuring the UV-Vis spectrum of the solution at 200-900 nm using Shimadzu spectrophotometer and the characteristic peaks were detected. FTIR analysis was performed using Perkin Elmer Spectrophotometer system, which was used to detect the characteristic peaks and their functional groups of S. johnstonii SNPs. To know the cytotoxic potentials, the brine shrimp bio-assay was performed. To reveal the anticancer potentials of Sargassum johnstonii SNPs, Trypan blue dye exclusion method was employed against DLA cell lines. On mixing the S. johnstonii aqueous extract with 1 mM AgNO3 solution, the colour of the solution changes from pale yellowish brown to dark brown colour indicates the presence of silver nanoparticles. The AgNP’s synthesised from aqueous extracts of S. johnstonii showed the optical peak at 430 nm with the absorption of 0.771. The results of FTIR analysis of S. johnstonii confirmed the presence of alkyl halides, aliphatic amines, alkynes, alcohols, amines and phenols with the peak values from 559.25 to 3401.82. The cytotoxic potential of S. johnstonii silver nanoparticles showed LC50 value at 656.89 μg/ml followed by aqueous extracts of S. johnstonii showed LC50 value at 805.45 μg/ml. The AgNPs of S. johnstonii showed their best growth inhibition activity with CTC50 value 293.66 μl/ml.

Keywords: Silver nanoparticles; Seaweeds; Sargassum.


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