Abstract

BIOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT POTENTIAL OF CYATHODIUM TUBEROSUM KASH COLLECTED FROM DIFFERENT ENVIRONMENTAL CONDITIONS

Arindam Ghatak, Palak Chaturvedi, Subhash Kudale, Neetin Desai*

ABSTRACT

Environment plays important role in determining the biochemical properties of plants. It also affects metabolite production which leads to change in morpho-physiological properties of plants. Cyathodium tuberosum Kash (Bryophyte) was evaluated for its antioxidant potential, total phenolics, chlorophyll, anthocyanin, carotenoid content, lipid peroxidation and amino acids content exposed to three different environmental conditions. The total chlorophyll, anthocyanin and carotenoid content were observed to be highest in the methanol solvent system from location 3 to that of ethanol and acetone. The radical scavenging potential and total phenolic content of methanol extracts collected from the location 3 showed high percent radical scavenging activity (60.06±3.8 %) and total phenolic content (5.37±0.01 mg/ml) than ethanol and acetone extracts. While ethanolic extract from location 1 showed maximum percent protection (76.88±4.5%) evaluated by TBARS assay. The polyphenol content (gallic acid, cathechol, vanillin) varied from location to location along with amino acid content (arginine, histidine, thyrosine, alanine) which were identified by HPLC analysis. The present study has revealed that methanol is the best solvent system for the extraction of phenolic compounds compare to ethanol and acetone. The findings of this study are in agreement with the other work on bryophytes that Cyathodium tuberosm can be used as bioindicator for environmental changes.

Keywords: Cyathodium tuberosum, antioxidant activity, DPPH activity, RP–HPLC, TBARS, Total phenolic content, Bryophytes, amino acids.