BIOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT POTENTIAL OF CYATHODIUM TUBEROSUM KASH COLLECTED FROM DIFFERENT ENVIRONMENTAL CONDITIONS
Arindam Ghatak, Palak Chaturvedi, Subhash Kudale, Neetin Desai*
ABSTRACT
Environment plays important role in determining the biochemical
properties of plants. It also affects metabolite production which leads
to change in morpho-physiological properties of plants. Cyathodium
tuberosum Kash (Bryophyte) was evaluated for its antioxidant
potential, total phenolics, chlorophyll, anthocyanin, carotenoid content,
lipid peroxidation and amino acids content exposed to three different
environmental conditions. The total chlorophyll, anthocyanin and
carotenoid content were observed to be highest in the methanol solvent
system from location 3 to that of ethanol and acetone. The radical
scavenging potential and total phenolic content of methanol extracts
collected from the location 3 showed high percent radical scavenging
activity (60.06±3.8 %) and total phenolic content (5.37±0.01 mg/ml)
than ethanol and acetone extracts. While ethanolic extract from
location 1 showed maximum percent protection (76.88±4.5%) evaluated by TBARS assay.
The polyphenol content (gallic acid, cathechol, vanillin) varied from location to location
along with amino acid content (arginine, histidine, thyrosine, alanine) which were identified
by HPLC analysis. The present study has revealed that methanol is the best solvent system
for the extraction of phenolic compounds compare to ethanol and acetone. The findings of this study are in agreement with the other work on bryophytes that Cyathodium tuberosm can be used as bioindicator for environmental changes.
Keywords: Cyathodium tuberosum, antioxidant activity, DPPH activity, RP–HPLC, TBARS, Total phenolic content, Bryophytes, amino acids.
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