ISOLATION AND CHARACTERIZATION OF HEAVY METALS AND ANTIBIOTIC RESISTANT BACTERIA FROM ENVIRONMENTAL SAMPLES
S.M. Gopinath*, Sheetal, Suneetha T.B, Amar Shankar
ABSTRACT
In current years toxic heavy metal pollution has become one of the
most critical environmental problems. In the present study, screening,
isolation and characterization of heavy metal and antibiotic resistant
bacteria from different environmental samples was carried out. Those
bacterial isolates which shows >512 μg/ml. minimum inhibitory
concentration were examined for antibiotic resistance activity.
Antibiotics such as Ciprofloxacin, tetracycline, amoxicillin and
erythromycin were taken for antibiotic resistance activity.
Concentration of antibiotics were taken >16 to >64 μg/ml. Then
characterization of selected bacterial isolate was carried out by 16S
rDNA technique. The bacteria which has more resistivity towards both
heavy metal and antibiotics that was identified by 16S rDNA
sequencing as Pseudomonas aeruginosa and is the most heavy metal
and antibiotic tolerant bacteria found in the environment.
Keywords: Heavy metals, MIC, 16S rDNA.
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