EFFECT OF PHYSICAL MUTAGENESIS ON THE ABILITY OF STAPHYLOCOCCUS AUREUS IN STAPHYLOKINASE PRODUCTION
Hameed M. Jasim, Riad A. Dellol and Alaa S. Hamzah
ABSTRACT
This study included collecting isolate S. aureus, a total of (200) clinical
samples were collected from two clinical sources were (100) samples
of urine and (100) samples from skin infections. Staphylokinase
production by S.aureus carried out , ability of (54) isolates S.aureus
were tested to staphylokinase production in selective medium plasma
agar and the results showed all isolates were produced staphylokinase
in different levels and appered zone of lysis on plasma agar and chose
efficient producing isolate was (S.aureus A15) for further experiments.
Enhanced staphylokinase production by UV mutated (S.aureus A15)
achieved after exposure fresh culture of S.aureus A15 for UV
irradiation (254nm) for 30 second under sterile conditions and the results appeared two
mutated isolates were more production comparison with wild type. Genomic DNA of the all
isolates S.aureus before and after mutagenesis were extracted by boiling method, and
amplification of Sak gene by PCR carried out by specific primers and size of staphylokinase
gene was about 400bp.
Keywords: S. aureus, staphylokinase, S.aureus A15.
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