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Abstract

PREVALENCE AND ANTIBIOGRAM OF ESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTIONS IN A TERTIARY CARE HOSPITAL

Dr. Sunil Kumar Biradar*1, Dr. Amaresh Nigudgi1, Dr. Praveen Kumar Doddamani2

1Department of Microbiology, Mahadevappa Rampure Medical college, Gulbarga, 585105, India.
2Department of Microbiology, Mediciti institute of medical sciences, Ghanpur village, Medchal Mandal, R.R district, Andhra Pradesh, 501401, India.

ABSTRACT

One of the most common bacterial infections seen in clinical practice particularly in developing countries are the Urinary tract infection (UTIs). Most common organism responsible for UTI is E.coli. Despite the wide availability of antimicrobial therapy, UTI still remains a major health hazard. This was a retrospective study conducted in a tertiary care hospital to know the prevalence and antibiogram of E. coli. Samples received include mid-stream clean catch urine, suprapubic aspirate and from Foley’s catheter. All the plates were inspected for growth and the isolates were identified by observing colony morphology, Gram-stain characteristics and relevant biochemical tests. The isolates were tested for their antimicrobial susceptibility and the results were interpreted by modified Kirby Bauer disc diffusion method, according to the guidelines of Clinical and Laboratory Standards Institute. Out of 704 samples tested, 438 (62.21%) were positive for E.coli. E.coli was highly resistant to Ampicillin (91.7%) followed by Ofloxacin (66.6%) and least resistance was seen with Imipenem (2.73%) followed by Piperacillin-tazobactam (21.6%) and Amikacin (21.9%). Knowledge of prevalence and antimicrobial susceptibility pattern of E. coli will help in selecting an appropriate antibiotic for empirical therapy. Formulation of hospital infection control committee and strict adherence to the guidelines of the committee will help in preventing the emergence of drug resistance.

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