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Abstract

BIOFABRICATION OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF OCIMUM SANCTUM L.

Naureen S. Khan, Ashwini K. Dixit* and Rajendra Mehta

ABSTRACT

Bio-Nanotechnology has gained an unavoidable attention because of the fiery properties of nano particles, this technology is emerging as a blazing area of present day research. Keeping in view the aforementioned facts in the present study phytofabrication has been done to synthesize silver nanoparticles by using the aqueous leaf extract of Ocimum sanctum L. (OLE) following an ecofriendly route via the Biological method. As this plant is having great medicinal potential, an attempt has been made to confirm whether the Ocimum extract & AgNPs aggregate can enhance the drug value of the plant. As a prerequisite for the above mentioned study biofabrication of Silver nanoparticles using natural reducing agents from (OLE) was done and were characterized by UV-Vis spectroscopy, (Scanning Electron Microscopy) SEM and Fourier Transform Infrared spectroscopy (FTIR).The synthesis of Ag NPs was confirmed by getting the SPR at~400nm by performing UV- VIS spectroscopy, size and shape of the synthesized Nanoparticles was determined by SEM, found to be spherical in shape with an average size of 250nm to 500nm. And the FTIR analysis revealed the presence of amines and phenolic compounds that act as a strong capping and stabilizing agent. This study offers a simple, cost effective, energy saving and green route of Ag NPs synthesis, used for effective drug preparation thereby serving the mankind and sounds to be a vital step in ebbing the pollution.

Keywords: Ocimum sanctum, Biofabrication, Ag Nanoparticle, SEM, FTIR.


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