DETECTION HIGH RISK OF HUMAN PAPILLOMA VIRUS GENOTYPE (16/18) IN IRAQI WOMEN PATIENTS WITH CERVICAL CARCINOMA BY USING CHROMOGEN - INSITU HYBRIDIZATION (CISH) TECHNIQUE
*Athraa Zaidan Hassan, Assist Prof. Dr. Sajaa J. AL-Khalidi, Dr. Ali Hussein Mohammed Ali AL- Khafaji and Dr. Basher S. Mohammed
ABSTRACT
AIM: To investigate the association between human papilloma virus
(HPV) infection and cervical cancer among Iraqi women patients.
Method: To determine the relationship between HPV and cervical
carcinioma, a retrospective study was done. This study was carried out
on 30 patients with hisopathologically confirmed primary cervical
cancer. Samples were collected from each patients, as well as ten (20)
cervical tissues from control individuals with no cancer. Chromogen
In situ hybridization (CISH) was used to detect HPV DNA (HPV 16
/18 DNA CISH in cervical tissues. Results: HPV 16/18 was detected
in 26/30 (86.7%) tumor sample while negative cases in 4/30 (13.3%)
of tumor sample. and HPV 16/18 was detected in 5/20(25.0%) of
cervical control group while negative cases in 15/20 (75.0%) of
cervical control group. Conclusion: Our results suggest that cervical
HPV infection is common in patients with carcinoma of the cervix with HPV16 / 18 being the
most prevalent type in Iraqi women patients. HPV infection may play a role in cervical
carcinogenesis
Keywords: cervical cancer, HPV, chromogen in situ hybridization.
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