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World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
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Abstract

DETECTION HIGH RISK OF HUMAN PAPILLOMA VIRUS GENOTYPE (16/18) IN IRAQI WOMEN PATIENTS WITH CERVICAL CARCINOMA BY USING CHROMOGEN - INSITU HYBRIDIZATION (CISH) TECHNIQUE

*Athraa Zaidan Hassan, Assist Prof. Dr. Sajaa J. AL-Khalidi, Dr. Ali Hussein Mohammed Ali AL- Khafaji and Dr. Basher S. Mohammed

ABSTRACT

AIM: To investigate the association between human papilloma virus (HPV) infection and cervical cancer among Iraqi women patients. Method: To determine the relationship between HPV and cervical carcinioma, a retrospective study was done. This study was carried out on 30 patients with hisopathologically confirmed primary cervical cancer. Samples were collected from each patients, as well as ten (20) cervical tissues from control individuals with no cancer. Chromogen In situ hybridization (CISH) was used to detect HPV DNA (HPV 16 /18 DNA CISH in cervical tissues. Results: HPV 16/18 was detected in 26/30 (86.7%) tumor sample while negative cases in 4/30 (13.3%) of tumor sample. and HPV 16/18 was detected in 5/20(25.0%) of cervical control group while negative cases in 15/20 (75.0%) of cervical control group. Conclusion: Our results suggest that cervical HPV infection is common in patients with carcinoma of the cervix with HPV16 / 18 being the most prevalent type in Iraqi women patients. HPV infection may play a role in cervical carcinogenesis

Keywords: cervical cancer, HPV, chromogen in situ hybridization.


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