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Abstract

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF PROTEINS ISOLATED FROM MUNTINGIA CALABURA PLANT ROOT

Mohamed Azmathulla Khan Y, Dinesha Ramadas*, Subhas Chandrappa Mundasada, Santosh Kumar N, Harsha R Kashyap, Chikkanna D

ABSTRACT

Objective: To investigate the in vitro, Antioxidant and Antiinflammatory activity of the proteins isolated from Muntingia Calabura plant root. Methods: Proteins of the extract was isolated by Ammonium sulphate protein precipitate method. In vitro antioxidant studies were carried out for the Muntingia Calabura root proteins using superoxide radical scavenging method and anti-inflammatory studies was done using membrane stabilization assay were performed for Muntingia Calabura root proteins. Results: The superoxide radical scavenging ability of the proteins was compared with standard antioxidant like BHA and Ascorbic acid at a maximum dosage of 8μg, where Muntingia Calabura root protein showed a maximum inhibition of 71% and BHA and Ascorbic showed 65 and 60% respectively. The anti-inflammatory activity of Muntingia Calabura root proteins were compared with the standard drug Diclofenac sodium, where at a

Keywords: dosage of 1000?g/ml, plant root protein showed maximum inhibition where as the drug Diclofenac Sodium showed at a dosage of 200?g/ml. Conclusion: The results of the present study concluded that the Muntingia Calabura root proteins possess significant anti


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