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Abstract

EXTRACTION, PURIFICATION, CHARACTERIZATION AND METAL ION CONCENTRATION OF PAPAIN FROM CARICA PAPAYA L.

Joslin D., Gowthami R., Jenitta Emima Packiyam E., Rama Bhat P.*, Jayadev K. and Shrinidhi Shetty

ABSTRACT

Carica papaya are rich in papain which shows extensive proteolytic
activity towards proteins, short- chain peptides, amino acid, esters and
amide links and is applied extensively in the fields of food and
medicine. In the present study it was the goal to investigate the papain
enzyme production and to test the enzyme activity under different
conditions like pH, temperature and different metal ion concentration.
The solution of papain exhibited an absorption maximum at a
wavelength of 280nm. The enzyme activity of purified papain was of
0.3 U/ml by dialysis membrane and in case of ion exchange
chromatography it was 0.24U/ml. The rate of enzyme catalysed
reactions generally increases with temperature. In the present study the optimum enzyme
activity was seen at the temperature of 65⁰C and enzyme has shown its maximum activity at
pH 6.5, above and below this pH, the activity declines. Enzyme activity was found more with
Ca2+ and K+ of the metal ions used.

Keywords: Carica papaya, Metal ions, Dialysis, Ion-exchange chromatography.


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