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Abstract

IN-VITRO AND IN-VIVO STUDY ON BACILLUS CEREUS ISOLATED FROM DIFFERENT FOODS SAMPLES

*Dhuha, A. K. and Habeeb, S. N.

ABSTRACT

140 food samples included (rice, infant food, flour, dairy products, meat, milk powder and spices) were collected randomly from restaurants, food stores and various sale points in the local markets of the Hilla city during the period of November 2014 to April 2015 in attempt to isolate of Bacillus cereus from those samples and detection the prevalence of diarrheal (hbl and nhe) and emetic (ces) enterotoxins genes by using Polymerase Chain Reaction (PCR). All bacterial isolates were identified by traditional methods represented by cultural, microscopic and biochemical examinations. The results showed that 56 (40%) of samples revealed positive growth for Bacillus cereus. Polymerase Chain Reaction technique (PCR) was used to detect the ability of bacterial isolates to produce two types of enterotoxins, diarrheal and emetic enterotoxins. The results showed that 4(7.14 %) of isolates harboure hblA gene, 16 (28.57%) hblC, 5(8.92 %) hblD, 46(82.14%) nheA, 24(42.85%) nhe B, 26 (46.42%) nheC and 3(5.35%) of the isolates harboure Ces gene. On the other hand, through using PCR it was found that nhe gene was present in all isolates, while hbl gene was found to vary among the diagnosed isolates. Moreover, The Ces gene was found only in isolates being obtained from rice samples, while it was not found in other types of samples. Orally treatment of mice with B. cereus cells and its toxins resulted in clinical signs represented by body weight losses and slow movement or drowsiness as compared with the control group.

Keywords: B. cereus, diarrheal toxin, emetic toxin, PCR, MYP, Agarose gel electrophoresis, nhe, hbl, mice.


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