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Abstract

OXIDATIVE STATE DISRUPTION OF CATALASE ACTIVITY BY SILVER NANOPARTICLE ON LIVER OF PREGNANT SWISS ALBINO MICE AND THEIR FOETUSES

Jyoti Prakash Pani*, Sankarsan Pani

ABSTRACT

Catalase enhances fermentation of oxidanyl into water and oxygen. The zymotic agent is declared as a universal protector of cell society from oxidative stress which causes damage and reflects a disproportionate relationship between the systematic manifestations of reactive oxygen species and a biological system’s ability. The present study aimed to estimate the disrupted Catalase activity on AgNPs treated liver tissue of pregnant Swiss albino mice and their foetuses. Pregnant mice were given AgNPs at a dose of 5, 10, 15 and 20mg/kg/day from Gestational Day (G.D.) 7-9. 50 foetuses were collected from 30 mothers on G.D. 18 of both groups as well as control. Liver of those 30 mothers and 50 foetuses were collected following dissection of foetuses and mothers, liver tissue were minced by adding 10 times normal saline (1:10 dilution). Catalase standard curve graph was plotted by Oxiselect TM Catalase activity assay protocol at 540 nm. After centrifuge, supernatant of control & treated samples were exposed to Worthington assay at 240-300 nm. Optical density is evaluated by UV-VIS spectrophotometer and relative concentration (U/ml) of Catalase is calculated by over plotting for comparison. In order to decompose hydrogen peroxide, the activity of Catalase is gradually increased (1:2.00664= ratio of successive relative concentration) in treated groups and was found highly significant at 180 and 720 second time interval whereas the same activity is found parallel or maintained in control group. (1:2) with chronological decrease in optical density and increase in relative concentration Catalase activity disrupts due to oxidative stress.

Keywords: Oxidative damage, Hydrogen peroxide, Catalase, Oxidative stress, Swiss albino mice pups.


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