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Abstract

U.V. SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF FLUCONAZOLE

Yadav Anandkumar*, Sonali Kapse-Mistry, Pramod Kadu

ABSTRACT

A rapid, simple, selective and precise U.V. Spectrophotometric method for estimation of Fluconazole content has been developed. The spectrophotometric detection was carried out at maximum absorption wavelength 260.8nm in water. Linearity was observed at concentration range of 25 to 500μg/ml with correlation coefficient of 0.999. LOD and LOQ were found to be 1.59 and 4.82μg/ml. The developed method was validated according to ICH guidelines for linearity, precision, accuracy, robustness, LOD and LOQ.

Keywords: U.V. Spectroscopy, Fluconazole, ICH guideliness, Validation.


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