WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

DNA FRAGMENTATION INDUCTION USING HYDROGEN PEROXIDE-A COMPARATIVE IN VITRO STUDY ON SEMEN FROM DAIRY AND BEEF BULL

*Sabina Valeanu, Drugociu Dan, Petru Rosca

Division of Reproduction, Department of Clinical SciencesUniversity of Agricultural Science and Veterinary Medicine IASI, Romania.

ABSTRACT

Aim of study: to investigate the extent to which spermatozoa from two different breed bulls showed a different sensitivity to oxidative stress consecutive hydrogen peroxide (H2O2) treatment. Material and methods: the DNA fragmentation index was determined using the sperm chromatin structure assay (SCSA), which assesses the susceptibility of sperm deoxyribonucleic acid (DNA) to acid-induced denaturation using the metachromatic dye acridine orange (AO). Three different experiments were performed for each of two bulls, the meanąSD being determined. Results: the spermatozoa incubated for 6 hours, revealed a dose-dependent increase in the DNA lesions expressed as DNA fragmentation index (DFI%), significant from a concentration of 150 mM, p≤0.03 for beef bulls and p≤0.01 for dairy bulls. The highest percent of spermatozoa with damaged DNA, 20.58% (meanąSD) for dairy bull respectively 21.97% (meanąSD) for beef bull was obtain after the treatment with 300 mM H2O2 and incubation for 6 hours. Conclusions: Dairy bull spermatozoa seem to be more sensitive to DNA fragmentation induction using H2O2 comparative with beef bull spermatozoa.

Keywords: DNA fragmentation; bull; SCSA, hydrogen peroxide.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More