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Abstract

UV METHOD FOR DETERMINATIONIN OF METHOTREXATE TABLET DOSAGE FORM

Nita Thamke*, Mukesh Mohite, Pratiksha Shinde and Pooja Hulle

ABSTRACT

Two simple, precise and economical UV methods have been developed for estimation of Methotrexat in bulk formulation. Method A involves measurement of UV absorbance in Zero order derivative at 374nm. Method B deals with area under curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 372-376nm. The drug follows Beer- Lambert’s law in the concentration range of3-10ug/ml in both the methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed methods can be successfully applied for estimation of Methotrexatin routine analytical work.[1,2,3]

Keywords: Methotrexat, Zero order derivative, Area Under Curve method (AUC), UV Spectrophotometer.


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