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Abstract

GC-MS ANALYSIS OF ALBIZIA LEBBECK BENTH

Md. Nazneen Bobby, Wesely Edward Gnanaraj, Johnson Marimuthu Alias Antonisamy*, Anto Arockia Raj Adaikalam, Vinnarasi Jamesraj

ABSTRACT

Objective: The present study was aimed to characterize the chemical
profile present in the petroleum ether, metahnolic, ethylacetate extract
of leaves of Albizia lebbeck Benth by using GCMS analysis and
predict their biological activity using PASS. Methods: 10 g powdered
sample is soaked with 30 mL methanol, petroleum ether and ethyl
acetate overnight and filtered through an ash less filter paper with
sodium sulphate (2 g). The extract is concentrated to 1 mL by bubbling
nitrogen into the solution. 2 μL of the extracts of leaves of A. lebbeck
were employed for GC-MS analysis. The detection employed the NIST
Ver.2.0-Year 2005 library and Wiley library. The biological activities
are predicted based on Dr. Duke’s Phytochemical and Ethnobotanical
Databases and Pass prediction. Results: The petroleum ether and
metahnolic, extract of A. lebbeck leaves revealed the presence of 23
different compounds whereas ethyl acetate extract of A. lebbeck showed the presence of 25
different compounds. PASS analysis predicted various kinds of biological properties for the
identified compounds. Conclusion: It paves the way for the development of several treatment
regimens based on different extracts. Further work is needed to isolate and identify these
bioactive compounds.

Keywords: GC-MS; PASS; Phytochemistry; Bio-efficacy.


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