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Abstract

ANALYTICAL STUDIES OF PROTEASE EXTRACTED FROM AZADIRACHTA INDICA

Amit Gupta* and Sushama R. Chaphalkar

ABSTRACT

Investigations have been carried out on proteases extracted from Azadirachta indica using variable concentration of PBS (phosphate buffered saline, ranging from 3 to 9) buffer in order to estimate the total protein and protease content. The results showed that Azadirachta indica showed higher protein (PH 5 to 9 using Tris HCl and ice cold acetone) and protease (using casein and citrate buffer) content in case of pH 6 to 8. Overall, the results showed that protease activity of leaves extracted from Azadirachta indica may be responsible for wound healing properties.

Keywords: Proteases; Azadirachta indica; protein; protease.


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