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Abstract

PREPARATION OF POLYVALENT DIAGNOSTIC ANTISERA FOR SALMONELLA SEROVARES TYPHI AND TYPHIMURIUM

Ameer S. Y. AL-Edhari*, Ala H. Haider, Habeeb S. Naher

ABSTRACT

Polyvalent diagnostic antisera for S.Typhi and S. Typhimurium have
been prepared during this study according to protocol being suggested
by Florida State University. Salmonella isolates were received from
laboratory of veterinary College of AL-Qadissya University.
Preparation of heat killed s antigen for each isolates were prepared and
injected in three groups of laboratory animals (rabbits). The first and
second groupwere injected with different antigens in four successive
doses with an interval time of three to four days, while the third group
was injected with normal saline and used as control. After the last
fourth injection, 10 ml of blood was collected from animals by heart
puncture then serum has been separated by centrifugation. The
separated antisera was tested with live and killed Salmonella antigens using the agglutination
test. The results were highly positive and specific. To determine the purity of prepared
antisera, it cross reaction with live antigens of some species belong Entrobactrecea
represented by E.coli, Klebsiella Spp. and Protus Spp. The results indicated that there is no
considerable reactions.

Keywords: S. Typhi, S. Typhimurium, E.coli, Klebsiella Spp. and Protus Spp.


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