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Abstract

SEMEN PREPARATION TECHNIQUES AND ITS COMPARATIVE ANALYSIS FOR ASSISTED REPRODUCTIVE TECHNOLOGY (ART)

Srividhya.M1, Krishnanand2, Sundara Mahalingam1, Akilan.C.A1,  Shanil DevP.V1

1Department of Biotechnology, Karpaga Vinayaga College of Engineering, Kanchipuram District, India.
2Sree Sastha Institute of Engineering and Technology, Chennai. India.

ABSTRACT

Infertility can be diagnosed when the couples have not achieved pregnancy after a year or more of normal coitus.In times past, the women were always blamed; but in recent years, it has been shown that male partner (25%), female partner (25%) and factors affecting both account for the remaining 50% cases. In order to treat infertility, many Assisted reproductive Techniques are in practice. The success rate of these techniques depends upon the preparation of the maternal semen sample. We assessed the quality of ten semen samples. Those samples with different semen parameters are then prepared for ART. We compared four major semen preparation techniques with three different rpm and analyzed the potential technique based on the motile sperm count (millions/ml). On the whole, Density Gradient Centrifugation (DGC) is the best technique for any kind of semen sample. Due to the cost effectiveness of Swim Up technique, it can be used when infertility is only with female. The Semen wash and Swim Down techniques are comparatively produce less potential sperms.

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