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Abstract

ANTIBACTERIAL ACTIVITY OF CRUDE METHANOLIC EXTRACT OF MARINE BROWN ALGA LOBOPHORA VARIEGATA (J.V.LAMOUROUX)

Thennarasan S. and Murugesan S.*

ABSTRACT

Seaweeds are the nature most complete and balanced nutrient source; they are potentially prolific source of highly secondary metabolites that leads to development of new pharmaceutical agent. The present study was carried out for the antimicrobial activity at various concentration ranging from 250 to 1000 μg/mL of the marine brown alga Lobophora variegata and were tested against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Salmonella typhi by disc diffusion method. The zone of inhibition was measured and compared with the standard streptomycin. At the concentration of 1000 μg/mL Klebsiella pneumoniae shows the highest activity (4.92 ± 0.002) and on the same concentration Bacillus subtilis shows the lowest activity (3.54 ± 0.001). This study established that methanol extracts of brown alga L. variegata was highly effective, against gram negative bacteria than gram positive bacterial pathogens at all the concentrations.

Keywords: Antibacterial activity, methanolic extract, Lobophora variegata pathogenic bacteria, MIC and MBC, Streptomycin.


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