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Abstract

UTILISATION OF OXIDATIVE COUPLING REACTIONS FOR THE ESTIMATION OF DOBUTAMINE

Mohan Krishna Loki Reddy*, Nallathambi Ramasamy, Sonte Sushmitha, Sedimbi Revathi Priyanka

Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhrapradesh, India.

ABSTRACT

Three simple spectrophotometric methods (A-C) for the assay of Dobutamine (DBU) in pure state and in formulations have been developed based on the oxidative coupling reaction of DBU, with N,Ndimethylamino- paraphenylenediamine (DMPD) in the presence of Chloramine–T (CAT) (method A), 4-aminophenazone (4-AP) in the presence of IO4 - (method B) and 3-methyl benzothiazolinone hydrazone (MBTH) in the presence of cerium IV (Ce IV) (method C). Regression analysis of Beer-Lambert plots showed good correlation in the concentration ranges 2.0-20.0 g/ml, 1.0-8.0 g/ml and 2.0-10.0 g/ml for methods A, B and C respectively. The results of analysis have been validated statistically and by recovery studies.

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