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Abstract

DETECTION OF BK POLYOMA VIRUS AMONG SUDANESE RENAL TRANSPLANTATION RECIPIENTS USING CYTOLOGY AND MOLECULAR BIOLOGY TECHNIQUES

Otba Mustafa Hassan, Magdi M Salih, Mohamed Siddig A. Elaziz, Shimaa Abdin Adam, Hisham Ali Waggiallah*

ABSTRACT

Objective: In the developing countries there is lack of recent information about detection of Polyoma virus using urine cytology and PCR, especially in Sudan. Polyoma virus-associated nephropathy is cause of graft dysfunction after kidney transplantation. The aim of this study is to compare between urine cytology and PCR-based assays for detection of BK Polyoma virus among Sudanese patients attending Sudanese Renal Transplantation Society Clinic. Material &Methods: one hundred and fifty renal transplant recipients attending Sudanese Renal Transplantation Society Clinic, both gender were included different age groups, patient were subjected to many types of immunosuppressive drugs, and different transplant duration. Urine specimen was collected, centrifuged and prepared for cytology according to Papanicolaou method. The remained deposit was prepared for PCR technique, extraction was done using guanidine technique. Results: The 150 patients 97 (64.67%) were males and 53(35.33%) were females. Most of the study groups were transplanted for duration less than 25 years. Urine cytology showed 5 (3.33%) nuclear inclusions associated with BK polyoma virus infection. whereas PCR showed 11 (7.33%) positive for BK polyoma virus DNA.) Conclusion: PCR was more sensitive than urine cytology for the detection of BK Polyoma virus in renal transplant patients. However Urine cytology is a simple, rapid, and cost-effective method.

Keywords: BK Polyoma virus, renal transplantation, PCR, Cytology.


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