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Dr. Muhammad Baqir MR Fakhrildin
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Abstract

EFFECT OF HONEY BEE AND COQ10 SUPPLEMENTED TO CRYOPRESERVATION SOLUTION ON FREEZE-THAWED HUMAN SPERM PARAMETERS

Mohammad Hassan Abdullah, Dr. Mohammad Oda Selman*, and
Dr. Muhammad Baqir M-R Fakhrildin

ABSTRACT

Background: Cryopreservation techniques highly developed field of Assisted Reproductive Technologies (ARTs) to help infertile couples to overcome infertility. Coenzyme Q10 is a vitamin – like substance is present in most eukaryotic cell, and produced in the mitochondria primarily. Objective: To study the effects of honey bee supplemented to freezing solution and COQ10 supplemented to thawing solution on sperm parameters during cryopreservation and cycle of human spermatozoa. Materials and Methods: One hundred and eight males were participated in this study. The mean age for all males included in this study was (29.94 ± 0.65) years and duration of infertility was 2-14 years. Semen samples were collected and seminal fluid analysis was done according to WHO (2010). Each semen sample was divided into three aliquots. The first group contain 1mL from semen sample enriched with 0.7 mL of cryopreservation solution only, while the other two groups contain low concentration 5% HB and high concentration 10% HB supplied to cryopreservation solution. Results: There was significant reduction (P<0.05) in the sperm concentration, significant improvement (P<0.05) in the percentages of sperm motility and morphologically normal sperm when compared to control group. Present study appeared that the direct swim-up technique resulted in significantly (P<0.05) better results for sperm parameters than the swim-up technique from all types of ejaculates classified according to male infertility factors. The study proved that there was significant (P<0.05) improvement in sperm parameters. Conclusion: Combination of 10% HB and 10 M COQ10 obtained best results for human sperm parameters post-thawing compared to other groups. Best technique in this study direct swim-up technique compared with centrifuge technique.

Keywords: cryopreservation, honey bee, Coenzyme Q10.


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