WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR GLYCYRRHIZIN AND ITS APPLICATION FOR HERBAL ANTIACNE GEL

Pratibha Nand*, Sushma Drabu, Rajinder K Gupta

ABSTRACT

Objective: Number of analytical techniques are available for estimation of glycyrrhizin in extracts and herbal formulations. To our knowledge, there is no specific HPTLC (high-performance thin layer chromatography method) for quantification of glycyrrhizin in herbal gel compositions. In the present study HPTLC method was developed, validated and used for fingerprinting of glycyrrhizin in anti-acne formulation. Methods: Physicochemical, phytochemical and antibacterial potential of Glycyrrhiza glabra was assessed. A simple HPTLC method was validated. Method comprised application of 5 μl sample on 60GF254 plate and was placed in twin trough glass chamber (10 cm×10 cm) having solvent system of Pet ether: Benzene: Ethyl Acetate: Glacial Acetic Acid (4:8:2.8:0.2, v/v/v/v) and quantification was done using TLC scanner and in UV chamber at wavelength of 254 nm. Results: This system was found to give compact spots for glycyrrhizin (Rf value: 0.38 ± 0.02). A strong linear relationship between the peak area and concentration of glycyrrhizin was observed with correlation coefficient (r2 = 0.99021 ± 7.470210). The method was validated for precision, accuracy, recovery and robustness. Amount of glycyrrhizin present in herbal extract and gel formulation was quantified as (7.934 %) and (1.070 %) respectively at 254 nm. Conclusion: It was a simple, sensitive, precise and reproducible method and can be used for quality assurance of the herbal composition containing glycyrrhizin.

Keywords: Glycyrrhiza glabra, glycyrrhizin, HPTLC, validation, Gel.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More