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Abstract

PROFILING THE SECONDARY CHEMICAL CLASS OF IN VIVO MELIA COMPOSITA WILLD. LEAF USING ETHANOLIC FRACTION

G. Jahirhussain*, K. Kala, P. Ayyappan, V. Muniappan, V. Tamilselvan, P. Rajkumar

ABSTRACT

The present study was aimed to investigate the phytochemical components of Ethanolic in vivo leaf extract of Melia composita Willd. (Synonym: Melia dubia Hiern (not of Cav.)) member of Meliaceae by using GC-MS method. The chromatogram of in vivo leaf extract identified 33 phytochemicals as constituents with more than one peak area for 7 compounds. Quantitative analysis of the different compounds were performed on a GC Clarus 500 Perkin Elmer gas chromatograph equipped with Column: Elite-1 (100 % Dimethyl poly siloxane), 30m x 0.25 mm ID x 1.0 μ df at the oven temperature programme of 110°C -2 min hold up to 280°C at the rate of 5°C/min-9 min hold. 1μl of each sample was injected in triplicate splits and quantities represented relative area percentage as derived from the integrator. The major compounds identified from the in vivo leaf powder were Vitamin E with 35.46% peak area, followed by 3,7,11,15-Tetramethyl-2- hexadecen-1-ol (18.13%), Phytol (11.28%), 1-Monolinoleoylglycerol trimethylsilyl ether (8.88%), Caryophyllene (4.50%), Tetradecanoic acid (2.94%), 9,12,15-Octadecatrienoic acid, 2-[(trimethylsilyl)oxy]-1-[[(trimethylsilyl)oxy]methyl]ethyl ester, (Z,Z,Z)- (2.62%) and 2- Pentanone, 4- hydroxy-4-methyl- (2.28%). The other compounds were represented by less than 2% peak area.

Keywords: Gas chromatography-Mass Spectrometry, Phytochemical components, Melia composita Willd.


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