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Abstract

EFFECT OF 0.2 % CHITOSAN IN ENDODONTIC SMEAR LAYER REMOVAL: SEM STUDY

Shaheen Venghat* and Mithra N. Hegde

ABSTRACT

Background and Objectives: to evaluate the efficacy the effect of 0.2% chitosan in endodontic smear layer removal using the scanning electron microscope. Materials and Methods: twenty four single rooted mandibular premolars teeth with a single root canal and a closed apex were randomly divided into two groups of 12 teeth each. Cleaning and shaping of the canals were done using hand and rotary instrumentation up to size 50, 0.06 taper. All the specimens were irrigated using 3% hypochlorite between each file size. Group 1 (G1) specimens were irrigated with final flush of 17 % EDTA. Group 2 (G2) specimens were irrigated with final flush of 0.2% Chitosan. The teeth were sectioned longitudinally and prepared for evaluation by scanning electron microscope. Results: Data obtained from the study was subjected to statistical analysis using Mann Whitney test. 17 % EDTA and 0.02% Chitosan effectively removed smear layer from coronal and middle one third. There were no statistical significant difference in removal of smear layer between G1 and G2. Both the groups failed to remove smear layer completely from apical third. Interpretation and Conclusion: Under the limitations of the present study following conclusions were drawn, chitosan effectively removed smear layer from the coronal and middle third of the root canal in very less concentration. Hence, it can be used as an adjunct during instrumentation in efficient cleansing of the root canal system.

Keywords: Chitosan, rotary instrumentation, sodium hypochlorite, root canal system, scanning electron microscope.


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