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Abstract

FREE RADICAL SCAVENGING ACTIVITY, TLC, HPTLC AND GCMS ANALYSIS OF DRY FLOWER OF MICHELIA CHAMPACA LINN

Malathi S. and Dr. Ravindran Rajan*

ABSTRACT

Free radicals contribute to more than one hundred disorders in humans. Free radical scavenging activity was observed in both the methanol extract (M.E) and aqueous extract (A.E) of Michelia champaca respectively. In this study significant free radical scavenging activity was determined by evaluating the inhibition concentration (IC50) in each test. In 2, 2-diphenyl-1-picrylhydrazyl (DPPH) model the extract displayed potential free radicals scavenging activity with IC50 of M.E is 270.80μg/mL and A.E is 395.77μg/mL.The nitric oxide model displayed IC50 of 282.74μg/mL in M.E and 470.80μg/mL in A.E. While the superoxide ion model showed IC50 of 269.62 μg/mL and 421.15μg/mL respectively, for both methanol and aqueous extract when compared to standard ascorbic acid. The presence of phenol, flavonoid and total antioxidant in both the extract justifies the antioxidant potential of the plant which brings about its free radicals scavenging potential.TLC plate results showed the five spots in methanol and its Rf values were 0.230, 0.307, 0.538, 0.641 and 0.948.whereas in aqueous extract shows only three bands and its Rf values were 0.4,0.7 and 0.9. GC-MS analysis showed the presence of 12 different phytochemicals with Carbamazepine 10,11 epoxide found to be the compound with maximum peak percentage 35.88% in methanol extract. HPTLC results confirmed that the extract contained several potential active components such as phenols, flavonoids, saponins and terpenoids as the slides revealed multi-coloured bands of varying intensities. This study confirmed that the M. champaca had multipotential antioxidant and free radicals scavenging activities. Thus may be due to the cumulative effect of the phytochemicals present in the flower which genuinely designate them as a free radical scavenger.

Keywords: Medicinal plants; Michelia champaca, Free radical scavenging activity, Phytochemical, Antioxidants, HPTLC, GC-MS.


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