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Abstract

STUDYING EFFECT OF BOSWELLIA, BETA HYDROXY ACID (BBA) ON DOPAMINERGIC DIFFERENTIATION OF THE EMBRYONIC STEM CELLS EXPRESSING NURR1 AND GPX1 GENES

Sajjad Biglari, Mojtaba Safi*, Navid Pourzardosht, Mohammad Ebadi, MD, Saeid Ebadi

ABSTRACT

Introduction: In the studies, it has been mentioned that the main cause of Parkinson’s disease is specific loss of dopaminergic neurons in the Mesencephalon area. Pharmacotherapy has been the main treatment for Parkinson’s disease up to now but side effects of these drugs and temporary treatment of disease’s symptoms are of the main drawbacks of this therapeutic method. It has been recently shown that BBA increases length and number of neuron branches by affecting neural cells and it has been specified in the studies that one of the drugs with plant origin for neurodegenerative diseases in Iranian and Indian traditional medicine is Boswellia (Beta Hydroxy Acid (Bba) content). Considering the mentioned materials, the most important goal of the present research is to study effect of Boswellia (Beta Hydroxy Acid (Bba) content) on dopaminergic differentiation of the embryonic stem cells expressing nurr1 and gpx1 genes. Method: In the present research, cellular tests were used to differentiate embryonic stem cells of rats expressing nurr1 and gpx1 genes to dopaminergic neurons with function and EB construction protocol was used for differentiating between embryonic stem cells to dopaminergic neurons. Then, we evaluated expression of neural progenitor cells markers (Nestin) and markers of dopaminergic neurons (neuron Tau, TH, Nurr1, Pitx3) with Real time PCR quantitative technique at the end of the third, fourth and fifth stages. In the third and fifth stages, specific markers expression was studied in protein level with Immunocytochemistry method. Results: It was shown that concurrent expression of Nurr1 and GPX1 along with BBA treatment with concentration of 30nM had the highest effect on increase of neural and neuron markers and finally expression of specific markers of dopaminergic neurons. Discussion and conclusion: Results show that BBA had positive effect on differentiation of stem cells into dopaminergic cells and dopaminergic neurons markers in the studied groups were expressed and the highest expression of these markers was observed in the group with external genes treated with BBA.

Keywords: Boswellia, dopaminergic, stem cells, nurr1 ?gpx1.


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